A suite of ¹⁹F based relaxation dispersion experiments to assess biomolecular motions

URN to cite this document:: urn:nbn:de:bvb:355-epub-447743
DOI to cite this document:: 10.5283/epub.44774

Overbeck, Jan H. ; Sprangers, Remco ; Kremer, Werner

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Date of publication of this fulltext: 09 Feb 2021 13:17

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Abstract

Proteins and nucleic acids are highly dynamic bio-molecules that can populate a variety of conformational states. NMR relaxation dispersion (RD) methods are uniquely suited to quantify the associated kinetic and thermodynamic parameters. Here, we present a consistent suite of F-19-based CPMG, on-resonance R-1 rho and off-resonance R-1 rho RD experiments. We validate these experiments by studying ...