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Site-Specific Labelling of Native Mammalian Proteins for Single-Molecule FRET Measurements

Gust, Alexander ; Jakob, Leonhard ; Zeitler, Daniela M. ; Bruckmann, Astrid ; Kramm, Kevin ; Willkomm, Sarah ; Tinnefeld, Philip ; Meister, Gunter ; Grohmann, Dina


Human cells are complex entities in which molecular recognition and selection are critical for cellular processes often driven by structural changes and dynamic interactions. Biomolecules appear in different chemical states, and modifications, such as phosphorylation, affect their function. Hence, using proteins in their chemically native state in biochemical and biophysical assays is essential. ...


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