Go to content
UR Home

A rapid luciferase transfection assay for transcription activation effects and stability control of estrogenic drugs in cell cultures

Meyer, T. ; Koop, R. ; Angerer, E. von ; Schönenberger, H. ; Holler, E.



Abstract

A rapid assay system for measuring the potential of estrogenic drugs is introduced. Luciferase induction could be measured in estrogen-receptor-positive human MCF-7 breast cancer cells, which had been transfected with a novel luciferase reporter plasmid ERE luc. The minimal requirement was 1 h exposure to the inducing drug and 3.5 h of incubation after removal of the drug. The assay system was ...

plus


Owner only: item control page
  1. Homepage UR

University Library

Publication Server

Contact:

Publishing: oa@ur.de

Dissertations: dissertationen@ur.de

Research data: daten@ur.de

Contact persons