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Computerized determination of growth kinetic curves and doubling times from cells in microculture

URN to cite this document:
urn:nbn:de:bvb:355-epub-48314
DOI to cite this document:
10.5283/epub.4831
Reile, Herta ; Birnböck, Herbert ; Bernhardt, Günther ; Spruß, Thilo ; Schönenberger, Helmut
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Date of publication of this fulltext: 05 Aug 2009 13:48


Abstract

In this paper we describe the microcomputer-aided determination of cell proliferation kinetics and doubling times utilizing a crystal violet assay and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay in microtitration plates. The analysis of spectrophotometric data provides the doubling times at any time of incubation. Plots of doubling time versus time of incubation give ...

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