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PETER-assay: Combined Impedimetric Detection of Permeability (PE) and Resistance (TER) of Barrier-Forming Cell Layers

Urban, Florian ; Hajek, Kathrin ; Naber, Tobias ; Anczykowski, Boris ; Schäfer, Marcus ; Wegener, Joachim



Abstract

Epithelial and endothelial barrier function is typically studied in vitro by growing the cells of interest on permeable supports that are sandwiched between two fluid compartments. This setup mimics the physiological situation with the cell layer as the diffusion barrier at the interface between two chemically distinct fluids. Routinely, the barrier function is quantitatively described by two key ...

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