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RNA polymerase I (Pol I) passage through nucleosomes depends on Pol I subunits binding its lobe structure

Merkl, Philipp E. ; Pilsl, Michael ; Fremter, Tobias ; Schwank, Katrin ; Engel, Christoph ; Längst, Gernot ; Milkereit, Philipp ; Griesenbeck, Joachim ; Tschochner, Herbert


RNA polymerase I (Pol I) is a highly efficient enzyme specialized in synthesizing most ribosomal RNAs. After nucleosome deposition at each round of rDNA replication, the Pol I transcription machinery has to deal with nucleosomal barriers. It has been suggested that Pol I?associated factors facilitate chromatin transcription, but it is unknown whether Pol I has an intrinsic capacity to transcribe ...


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