The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans

URN to cite this document:: urn:nbn:de:bvb:355-epub-528607
DOI to cite this document:: 10.5283/epub.52860

Daiß, Julia L. ; Pilsl, Michael ; Straub, Kristina

; Bleckmann, Andrea

; Höcherl, Mona ; Heiss, Florian B.

; Abascal-Palacios, Guillermo

; Ramsay, Ewan Phillip ; Tlučková, Katarina ; Mars, Jean-Clement ; Fürtges, Torben ; Bruckmann, Astrid

; Rudack, Till ; Bernecky, Carrie ; Lamour, Valérie ; Panov, Konstantin

; Vannini, Alessandro

; Moss, Tom

; Engel, Christoph

License: Creative Commons Attribution 4.0
PDF - Published Version
(3MB)

Date of publication of this fulltext: 09 Sep 2022 12:41

Alternative links to fulltext:DOI Publisher Publisher

Details

Preview

Indexed in

Export bibliographical data

Abstract

Transcription of the ribosomal RNA precursor by RNA polymerase (Pol) I is a major determinant of cellular growth, and dysregulation is observed in many cancer types. Here, we present the purification of human Pol I from cells carrying a genomic GFP fusion on the largest subunit allowing the structural and functional analysis of the enzyme across species. In contrast to yeast, human Pol I carries ...