Insertion of Nanoluc into the Extracellular Loops as a Complementary Method To Establish BRET-Based Binding Assays for GPCRs

URN to cite this document:: urn:nbn:de:bvb:355-epub-532390
DOI to cite this document:: 10.5283/epub.53239

Grätz, Lukas ; Müller, Christoph ; Pegoli, Andrea

; Schindler, Lisa ; Bernhardt, Günther

; Littmann, Timo

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 21 Nov 2022 07:37

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Abstract

Luminescence-based techniques play an increasingly important role in all areas of biochemical research, including investigations on G protein-coupled receptors (GPCRs). One quite recent and popular addition has been made by introducing bioluminescence resonance energy transfer (BRET)-based binding assays for GPCRs, which are based on the fusion of nanoluciferase (Nluc) to the N-terminus of the ...