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Reassociation of lactic dehydrogenase from pig heart studied by cross-linking with glutaraldehyde

Bernhardt, Günther ; Rudolph, Rainer ; Jaenicke, Rainer



Abstract

Crosslinking with glutaraldehyde was applied to the study of the assembly of lactate dehydrogenase from pig heart. To eliminate perturbations caused by excessive folding reactions, acid dissocn. was performed in the presence of 0.8M Na2SO4 at 0 Deg. Under optimum conditions complete crosslinking of the tetrameric enzyme was achieved in <2 min. Crosslinking during reconstitution proves the dimer ...

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