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Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaea

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Grünberger, Felix ; Jüttner, Michael ; Knüppel, Robert Michael ; Ferreira-Cerca, Sébastien ; Grohmann, Dina
Date of publication of this fulltext: 02 Nov 2023 16:20


Ribosomal RNA (rRNA) maturation in archaea is a complex multistep process that requires well-defined endo- and exoribonuclease activities to generate fully mature linear rRNAs. However, technical challenges prevented detailed mapping of rRNA processing steps and a systematic analysis of rRNA maturation pathways across the tree of life. In this study, we used long-read (PCR)-cDNA and direct RNA ...


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