Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaea

URN to cite this document:: urn:nbn:de:bvb:355-epub-549520
DOI to cite this document:: 10.5283/epub.54952

Grünberger, Felix

; Jüttner, Michael ; Knüppel, Robert Michael ; Ferreira-Cerca, Sébastien

; Grohmann, Dina

License: Creative Commons Attribution Non-commercial 4.0
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Date of publication of this fulltext: 02 Nov 2023 16:20

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Abstract

Ribosomal RNA (rRNA) maturation in archaea is a complex multistep process that requires well-defined endo- and exoribonuclease activities to generate fully mature linear rRNAs. However, technical challenges prevented detailed mapping of rRNA processing steps and a systematic analysis of rRNA maturation pathways across the tree of life. In this study, we used long-read (PCR)-cDNA and direct RNA ...