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Dilute Bicelles for Glycosyltransferase Studies, Novel Bicelles with Phosphatidylinositol

Patrick, Joan ; Alija, Mikel García ; Liebau, Jobst ; Pettersson, Pontus ; Metola, Ane ; Mäler, Lena



Abstract

Solution-state NMR can be used to study protein- lipid interactions, in particular, the effect that proteins have on lipids. One drawback is that only small assemblies can be studied, and therefore, fast-tumbling bicelles are commonly used. Bicelles contain a lipid bilayer that is solubilized by detergents. A complication is that they are only stable at high concentrations, exceeding the CMC of ...

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