Zusammenfassung
It has been postulated that the ubiquitous (beta alpha)(8)-barrel enzyme fold has evolved by duplication and fusion of an ancestral (beta alpha)(4)-half-barrel. We have previously reconstructed this process in the laboratory by fusing two copies of the C-terminal half-barrel HisF-C of imidazole glycerol phosphate synthase (HisF). The resulting construct HisF-CC was stepwise stabilized to Sym1 and ...
Zusammenfassung
It has been postulated that the ubiquitous (beta alpha)(8)-barrel enzyme fold has evolved by duplication and fusion of an ancestral (beta alpha)(4)-half-barrel. We have previously reconstructed this process in the laboratory by fusing two copies of the C-terminal half-barrel HisF-C of imidazole glycerol phosphate synthase (HisF). The resulting construct HisF-CC was stepwise stabilized to Sym1 and Sym2, which are extremely robust but catalytically inert proteins. Here, we report on the generation of a circular permutant of Sym2 and the establishment of a sugar isomerization reaction on its scaffold. Our results demonstrate that duplication and mutagenesis of (beta alpha)(4)-half-barrels can readily lead to a stable and catalytically active (beta alpha)(8)-barrel enzyme. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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