| Item type: | Article | ||||
|---|---|---|---|---|---|
| Journal or Publication Title: | Pfl�gers Archiv European Journal of Physiology | ||||
| Publisher: | SPRINGER HEIDELBERG | ||||
| Place of Publication: | HEIDELBERG | ||||
| Volume: | 442 | ||||
| Number of Issue or Book Chapter: | 6 | ||||
| Page Range: | pp. 821-827 | ||||
| Date: | 2001 | ||||
| Institutions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz Chemistry and Pharmacy > Institute of Pharmacy > Alumni or Retired Professors > Prof. Wiegrebe | ||||
| Identification Number: |
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| Keywords: | NITRIC-OXIDE-SYNTHASE; MESSENGER-RNA; RENOVASCULAR HYPERTENSION; GENE-EXPRESSION; MACULA DENSA; RAT-KIDNEY; INHIBITION; SPIRONOLACTONE; IDENTIFICATION; STIMULATION; angiotensin II; cyclooxygenase-2; kidney; prostaglandins; renin; spontaneously hypertensive rats (SHR) | ||||
| Dewey Decimal Classification: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy | ||||
| Status: | Published | ||||
| Refereed: | Yes, this version has been refereed | ||||
| Created at the University of Regensburg: | Yes | ||||
| Item ID: | 73487 |
Web of ScienceAbstract
Based on recent evidence that renin gene and cyclooxygenase-2 (COX-2) expression in the rat kidney cortex increase in parallel under a variety of conditions, this study aimed to characterize the causal linkage between COX-2 and renin expression. Therefore, we semi-quantitated renocortical renin and COX-2 gene expression when the renin-angiotensin system (RAS) was inhibited by the angiotensin II ...
Abstract
Based on recent evidence that renin gene and cyclooxygenase-2 (COX-2) expression in the rat kidney cortex increase in parallel under a variety of conditions, this study aimed to characterize the causal linkage between COX-2 and renin expression. Therefore, we semi-quantitated renocortical renin and COX-2 gene expression when the renin-angiotensin system (RAS) was inhibited by the angiotensin II (Ang II) AT1 receptor antagonist candesartan (15 mg/kg per day) and when COX-2 activity was blocked by celecoxib (20 mg/kg twice a day) in three rat strains (Sprague-Dawley, WKY and SHR) at ages of 5, 9 and 15 weeks. We observed that candesartan increased renin mRNA in all rats at all ages, the amplitude of stimulation being inversely related to age. Candesartan increased COX-2 mRNA in all three strains at 5 weeks, and in SID and WKY rats also at 9 weeks. In 9-week-old SUR and in 15-week-old rats of all three strains candesartan did not influence COX-2 mRNA levels. For all rat strains, strain-specific strong linear correlations existed between renocortical COX-2 and renin mRNA levels, both with and without candesartan treatment. The additional feeding of candesartan-treated rats with celecoxib did not change renin mRNA or COX-2 mRNA levels, whilst the renal excretion of sodium and renal cortical prostaglandin E-2 concentration decreased by 26% and 60%, respectively. In summary, these findings, obtained when the renin system was activated by AT(1) receptor blockade, indicate that Ang II is not required to stimulate COX-2 expression and that COX-2 activity is not required to stimulate renin expression. However, the renocortical expression of renin and of COX-2 appear to be highly coordinated under basal conditions and during inhibition of RAS, suggesting the existence of a common denominator for renin and COX-2 expression that remains to be elucidated.
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