Simultaneous determination of free and total metabolite concentrations in proteinaceous specimens by 1D 1H CPMG NMR

URN to cite this document:: urn:nbn:de:bvb:355-epub-785237
DOI to cite this document:: 10.5283/epub.78523

Reindl, Alexander ; Samol, Claudia ; Haerteis, Silke

; Zacharias, Helena U. ; Dettmer, Katja

; Oefner, Peter J.

; Gronwald, Wolfram

License: Creative Commons Attribution 4.0
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Date of publication of this fulltext: 29 Jan 2026 06:04

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Abstract

Nuclear magnetic resonance (NMR) spectroscopy is often used for the analysis of metabolites in proteinaceous biological specimens. However, the binding of metabolites to proteins impedes accurate quantitation of total metabolite concentrations by NMR, unless protein binding is disrupted by organic solvent precipitation, which increases variance and may result in the loss of volatile metabolites ...