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Reassociation of lactic dehydrogenase from pig heart studied by cross-linking with glutaraldehyde

Bernhardt, Günther and Rudolph, Rainer and Jaenicke, Rainer (1981) Reassociation of lactic dehydrogenase from pig heart studied by cross-linking with glutaraldehyde. Zeitschrift fuer Naturforschung, C Journal of Biosciences 36C (9-10), pp. 772-777.

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Abstract

Crosslinking with glutaraldehyde was applied to the study of the assembly of lactate dehydrogenase from pig heart. To eliminate perturbations caused by excessive folding reactions, acid dissocn. was performed in the presence of 0.8M Na2SO4 at 0 Deg. Under optimum conditions complete crosslinking of the tetrameric enzyme was achieved in <2 min. Crosslinking during reconstitution proves the dimer ...

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Item Type:Article
Date:1981
Additional information (public):CAN 95:217064 7-5 Enzymes 9001-60-9 Role: BIOL (Biological study) (subunit assembly of, kinetics of, of heart)
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie
Identification Number:
ValueType
1981:617064Other
Keywords:Heart, composition (lactate dehydrogenase of, subunit assembly of); Kinetics, reaction (of lactate dehydrogenase subunit assembly); Molecular association (of lactate dehydrogenase subunits, kinetics of); lactate dehydrogenase subunit assembly
Subjects:500 Science > 570 Life sciences
500 Science > 540 Chemistry & allied sciences
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Owner: Guenther Bernhardt
Deposited On:15 Jan 2009 16:24
Last Modified:05 Aug 2009 13:50
Item ID:5474
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