Reversible activation of NADPH oxidase in membranes of HL-60 human leukemic cells

URN to cite this document:: urn:nbn:de:bvb:355-epub-232725
DOI to cite this document:: 10.5283/epub.23272

Seifert, Roland ; Schultz, Günter

Preview

PDF
(434kB)

Date of publication of this fulltext: 25 Jan 2012 13:49

Alternative links to fulltext:Pubmed

Details

Preview

Export bibliographical data

Abstract

NADPH oxidase in membranes of undifferentiated and dimethylsulphoxide-differentiated HL-60 cells was activated by arachidonic acid (AA) in the presence of Mg2+ and a cytosolic cofactor (CF) found in differentiated HL-60 cells. Basal superoxide (O2-) formation was enhanced several-fold by addition of the stable GTP-analogue, guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S), prior to AA and was ...