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High performance anion exchange chromatography with pulsed amperometric detection (HPAEC–PAD) for the sensitive determination of hyaluronan oligosaccharides

Rothenhöfer, Martin, Grundmann, Marco, Bernhardt, Günther, Matysik, Frank-Michael and Buschauer, Armin (2015) High performance anion exchange chromatography with pulsed amperometric detection (HPAEC–PAD) for the sensitive determination of hyaluronan oligosaccharides. Journal of Chromatography B 988 (C), pp. 106-115.

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Abstract

High performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) was optimized for the analysis of oligosaccharides derived from the extracellular matrix component hyaluronan. Using this sensitive approach, the separation of oligosaccharides consisting of 2 (molecular weight ca. 0.8 kDa) up to 25–30 (molecular weight: ca. 9.5–11.4 kDa) disaccharide moieties was ...

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Item type:Article
Date:15 April 2015
Additional Information (public):Supplementary Material available
Institutions:Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer)
Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik)
Identification Number:
ValueType
10.1016/j.jchromb.2015.02.027DOI
Related URLs:
URLURL Type
http://www.sciencedirect.com/science/article/pii/S1570023215001269Publisher
Keywords:Bovine testicular hyaluronidase, Capillary electrophoresis, High performance anion exchange chromatography, Hyaluronan oligosaccharides, Pulsed amperometric detection, Turbidimetric hyaluronidase assay
Dewey Decimal Classification:500 Science > 540 Chemistry & allied sciences
600 Technology > 615 Pharmacy
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:28703
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