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High performance anion exchange chromatography with pulsed amperometric detection (HPAEC–PAD) for the sensitive determination of hyaluronan oligosaccharides

Rothenhöfer, Martin ; Grundmann, Marco ; Bernhardt, Günther ; Matysik, Frank-Michael ; Buschauer, Armin



Abstract

High performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) was optimized for the analysis of oligosaccharides derived from the extracellular matrix component hyaluronan. Using this sensitive approach, the separation of oligosaccharides consisting of 2 (molecular weight ca. 0.8 kDa) up to 25–30 (molecular weight: ca. 9.5–11.4 kDa) disaccharide moieties was ...

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