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Metagenomic analysis of the stool microbiome in patients receiving allogeneic SCT: Loss of diversity is associated with use of systemic antibiotics and more pronounced in gastrointestinal GvHD

Holler, Ernst ; Butzhammer, Peter ; Schmid, Karin ; Hundsrucker, Christian ; Koestler, Josef ; Peter, Katrin ; Zhu, Wentao ; Sporrer, Daniela ; Hehlgans, Thomas ; Kreutz, Marina ; Holler, Barbara ; Wolff, Daniel ; Edinger, Matthias ; Andreesen, Reinhard ; Levine, John E. ; Ferrara, James L. ; Gessner, Andre ; Spang, Rainer ; Oefner, Peter J.


Next-generation sequencing of the hypervariable V3 region of the 16s rRNA gene isolated from serial stool specimens collected from 31 patients receiving allogeneic stem cell transplantation (SCT) was performed to elucidate variations in the composition of the intestinal microbiome in the course of allogeneic SCT. Metagenomic analysis was complemented by strain-specific enterococcal PCR and ...


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