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Metagenomic analysis of the stool microbiome in patients receiving allogeneic SCT: Loss of diversity is associated with use of systemic antibiotics and more pronounced in gastrointestinal GvHD

Holler, Ernst, Butzhammer, Peter, Schmid, Karin, Hundsrucker, Christian, Koestler, Josef, Peter, Katrin, Zhu, Wentao, Sporrer, Daniela, Hehlgans, Thomas, Kreutz, Marina, Holler, Barbara, Wolff, Daniel, Edinger, Matthias, Andreesen, Reinhard, Levine, John E., Ferrara, James L, Gessner, Andre, Spang, Rainer and Oefner, Peter J. (2014) Metagenomic analysis of the stool microbiome in patients receiving allogeneic SCT: Loss of diversity is associated with use of systemic antibiotics and more pronounced in gastrointestinal GvHD. Biology of Blood and Marrow Transplantation 20 (5), pp. 640-645.

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Abstract

Next-generation sequencing of the hypervariable V3 region of the 16s rRNA gene isolated from serial stool specimens collected from 31 patients receiving allogeneic stem cell transplantation (SCT) was performed to elucidate variations in the composition of the intestinal microbiome in the course of allogeneic SCT. Metagenomic analysis was complemented by strain-specific enterococcal PCR and ...

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Item type:Article
Date:May 2014
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie)
Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang)
Identification Number:
ValueType
10.1016/j.bbmt.2014.01.030DOI
24492144PubMed ID
Keywords:Clinical allogeneic transplantation; GVHD; Metagenomics; Microbiome
Dewey Decimal Classification:500 Science > 500 Natural sciences & mathematics
500 Science > 570 Life sciences
600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Partially
Item ID:30496
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