Adjusting microbiome profiles for differences in microbial load by spike-in bacteria

URN to cite this document:: urn:nbn:de:bvb:355-epub-352481
DOI to cite this document:: 10.5283/epub.35248

Stämmler, Frank ; Glasner, J. ; Hiergeist, Andreas ; Holler, Ernst ; Weber, Daniela ; Oefner, Peter J.

; Gessner, Andre ; Spang, Rainer

Preview

PDF - Published Version
(1MB)

Date of publication of this fulltext: 20 Feb 2017 12:56

Alternative links to fulltext:Pubmed DOI

Details

Preview

Indexed in

Export bibliographical data

Abstract

Background: Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. However, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loads vary across specimens. Results: Here we suggest the spiking of exogenous bacteria into crude ...