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Adjusting microbiome profiles for differences in microbial load by spike-in bacteria

Stämmler, Frank, Glasner, J., Hiergeist, Andreas , Holler, Ernst, Weber, Daniela, Oefner, Peter J. , Gessner, Andre and Spang, Rainer (2016) Adjusting microbiome profiles for differences in microbial load by spike-in bacteria. Microbiome 4 (1), p. 28.

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Date of publication of this fulltext: 20 Feb 2017 12:56

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Abstract

Background Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. However, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loads vary across specimens. Results Here we suggest the spiking of exogenous bacteria into crude ...

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Item type:Article
Date:21 June 2016
Institutions:Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner)
Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie)
Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Statistische Bioinformatik (Prof. Spang)
Identification Number:
ValueType
27329048PubMed ID
10.1186/s40168-016-0175-0DOI
Keywords:Microbial load, Spike-in bacteria, 16S rRNA gene sequencing, Standardization, Microbiome profiling, Bacterial communities, Community analysis
Dewey Decimal Classification:600 Technology > 610 Medical sciences Medicine
Status:Published
Refereed:Yes, this version has been refereed
Created at the University of Regensburg:Yes
Item ID:35248
Owner only: item control page

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