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Adjusting microbiome profiles for differences in microbial load by spike-in bacteria

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Stämmler, Frank ; Glasner, J. ; Hiergeist, Andreas ; Holler, Ernst ; Weber, Daniela ; Oefner, Peter J. ; Gessner, Andre ; Spang, Rainer
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Date of publication of this fulltext: 20 Feb 2017 12:56


Background: Next-generation 16S ribosomal RNA gene sequencing is widely used to determine the relative composition of the mammalian gut microbiomes. However, in the absence of a reference, this does not reveal alterations in absolute abundance of specific operational taxonomic units if microbial loads vary across specimens. Results: Here we suggest the spiking of exogenous bacteria into crude ...


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