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Imaging in Biologically-Relevant Environments with AFM Using Stiff qPlus Sensors

URN to cite this document:
urn:nbn:de:bvb:355-epub-374153
DOI to cite this document:
10.5283/epub.37415
Pürckhauer, Korbinian ; Weymouth, Alfred J. ; Pfeffer, Katharina ; Kullmann, Lars ; Mulvihill, Estefania ; Krahn, Michael P. ; Müller, Daniel J. ; Giessibl, Franz J.
Date of publication of this fulltext: 19 Jun 2018 13:20


Abstract

High-resolution imaging of soft biological samples with atomic force microscopy (AFM) is challenging because they must be imaged with small forces to prevent deformation. Typically, AFM of those samples is performed with soft silicon cantilevers (k ≈ 0.1–10 N/m) and optical detection in a liquid environment. We set up a new microscope that uses a stiff qPlus sensor (k ≥ 1 kN/m). Several complex ...

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