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Imaging in Biologically-Relevant Environments with AFM Using Stiff qPlus Sensors

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Pürckhauer, Korbinian ; Weymouth, Alfred J. ; Pfeffer, Katharina ; Kullmann, Lars ; Mulvihill, Estefania ; Krahn, Michael P. ; Müller, Daniel J. ; Giessibl, Franz J.
Date of publication of this fulltext: 19 Jun 2018 13:20


High-resolution imaging of soft biological samples with atomic force microscopy (AFM) is challenging because they must be imaged with small forces to prevent deformation. Typically, AFM of those samples is performed with soft silicon cantilevers (k ≈ 0.1–10 N/m) and optical detection in a liquid environment. We set up a new microscope that uses a stiff qPlus sensor (k ≥ 1 kN/m). Several complex ...


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