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Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

Busch, Florian ; Rajendran, Chitra ; Heyn, Kristina ; Schlee, Sandra ; Merkl, Rainer ; Sterner, Reinhard



Zusammenfassung

Modern enzyme complexes are characterized by a high catalytic efficiency and allosteric communication between the constituting protein subunits. We were interested in whether primordial enzyme complexes from extinct species displayed a similar degree of functional sophistication. To this end, we used ancestral sequence reconstruction to resurrect the alpha and beta subunits of the tryptophan ...

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