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Viewing pre-60S maturation at a minute’s timescale

Zisser, Gertrude ; Ohmayer, Uli ; Mauerhofer, Christina ; Mitterer, Valentin ; Klein, Isabella ; Rechberger, Gerald N ; Wolinski, Heimo ; Prattes, Michael ; Pertschy, Brigitte ; Milkereit, Philipp ; Bergler, Helmut


The formation of ribosomal subunits is a highly dynamic process that is initiated in the nucleus and involves more than 200 trans-acting factors, some of which accompany the pre-ribosomes into the cytoplasm and have to be recycled into the nucleus. The inhibitor diazaborine prevents cytoplasmic release and recycling of shuttling pre-60S maturation factors by inhibiting the AAA-ATPase Drg1. The ...


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