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Quaternary Structure of the Tryptophan Synthase α-Subunit Homolog BX1 from Zea mays

Norris, Andrew ; Busch, Florian ; Schupfner, Michael ; Sterner, Reinhard ; Wysocki, Vicki H.



Abstract

BX1 from Zea mays (zmBX1) is an enzyme of plant secondary metabolism that generates indole for the synthesis of plant defensins. It is a homologue of the tryptophan synthase alpha-subunit, TrpA. Whereas TrpA itself is a monomer in solution, zmBX1 is dimeric, confirmed in our work by native MS. Using cross-linking and mutagenesis, we identified the physiological dimerization interface of zmBX1. We ...

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