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Isotope Ratio Outlier Analysis (IROA) for HPLC–TOFMS-Based Metabolomics of Human Urine

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Fadil, Fadi ; Samol, Claudia ; Berger, Raffaela S. ; Kellermeier, Fabian ; Gronwald, Wolfram ; Oefner, Peter J. ; Dettmer, Katja
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Date of publication of this fulltext: 28 Sep 2022 12:32


Metabolic fingerprinting by mass spectrometry aims at the comprehensive, semiquantitative analysis of metabolites. Isotope dilution, if successfully implemented, may provide a more reliable, relative quantification. Therefore, the C-13 labeled yeast extract of the IROA TruQuant kit was added as an internal standard (IS) to human urine samples measured in full-scan mode on a high-performance ...


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