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Label-free versus conventional cellular assays: functional investigations on the human histamine H1 receptor

Lieb, Sebastian ; Littmann, Timo ; Plank, Nicole ; Felixberger, Johannes ; Tanaka, Miho ; Schäfer, Thorsten ; Krief, Stéphane ; Elz, Sigurd ; Friedland, Kristina ; Bernhardt, Günther ; Wegener, Joachim ; Ozawa, Takeaki ; Buschauer, Armin


A set of histamine H-1 receptor (H-1 R) agonists and antagonists was characterized in functional assays, using dynamic mass redistribution (DMR), electric cell-substrate impedance sensing (ECIS) and various signaling pathway specific readouts (Fura-2 and aequorin calcium assays, arrestin recruitment (luciferase fragment complementation) assay, luciferase gene reporter assay). Data were gained ...


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