pH sensing in skin tumors: Methods to study the involvement of GPCRs, acid‐sensing ion channels and transient receptor potential vanilloid channels

URN to cite this document:

urn:nbn:de:bvb:355-epub-445544 Copy

DOI to cite this document:

10.5283/epub.44554 Copy

Schreml, Stephan ; Stolwijk, Judith A. ; Sauer, Lisa ; Ackermann, Kirsten ; Nassios, Anaïs ; Aung, Thiha ; Haerteis, Silke ; Bäumner, Antje J. ; Wegener, Joachim

Preview

License: Creative Commons Attribution Non-commercial No Derivatives 4.0 PDF - Published Version (1MB)

Date of publication of this fulltext: 26 Jan 2021 17:56

---

Alternative links to fulltext:DOIPublisher

---

Details

Item type:	Article
Journal or Publication Title:	Experimental Dermatology
Publisher:	Wiley
Volume:	29
Number of Issue or Book Chapter:	11
Page Range:	pp. 1055-1061
Date:	November 2020
Institutions:	Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener)
Projects:	DFG Grant number SCHR 1288/4‐3, DFG Grant number SCHR 1288/6‐1, DFG Grant number WE 2721/7‐3
Identification Number:	Value Type 10.1111/exd.14150 DOI
Keywords:	cell migration, melanoma, skin barrier, squamous cell carcinoma, wound healing
Dewey Decimal Classification:	500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine
Status:	Published
Refereed:	Yes, this version has been refereed
Created at the University of Regensburg:	Yes
Item ID:	44554

Preview

Export bibliographical data

ASCII CitationAtomBibTeXData Cite XMLDataCite XML OAIDublin CoreEndNoteHTML CitationJSON LDMETSMODSOpenAPCRDF+N-TriplesRDF+N3RDF+XMLReferReference ManagerSimple MetadataXMLxMetaDissPlus

---

Abstract

Solid tumors exhibit an inversed pH gradient with increased intracellular pH (pHi) and decreased extracellular pH (pHe). This inside‐out pH gradient is generated via sodium/hydrogen antiporter 1, vacuolar‐type H + ATPases, monocarboxylate transporters, (bi)carbonate (co)transporters and carboanhydrases. Our knowledge on how pHe‐signals are sensed and what the respective receptors induce inside ...

Abstract

Solid tumors exhibit an inversed pH gradient with increased intracellular pH (pHi) and decreased extracellular pH (pHe). This inside‐out pH gradient is generated via sodium/hydrogen antiporter 1, vacuolar‐type H + ATPases, monocarboxylate transporters, (bi)carbonate (co)transporters and carboanhydrases. Our knowledge on how pHe‐signals are sensed and what the respective receptors induce inside cells is scarce. Some pH‐sensitive receptors (GPR4, GPR65/TDAG8, GPR68/OGR1, GPR132/G2A, possibly GPR31 and GPR151) and ion channels (acid‐sensing ion channels ASICs, transient receptor potential vanilloid receptors TRPVs) transduce signals inside cells. As little is known on the expression and function of these pH sensors, we used immunostainings to study tissue samples from common and rare skin cancers. Our current and future work is directed towards investigating the impact of all the pH‐sensing receptors in different skin tumors using cell culture techniques with selective knockdown/knockout (siRNA/CRISPR‐Cas9). To study cell migration and proliferation, novel impedance‐based wound healing assays have been developed and are used. The field of pH sensing in tumors and wounds holds great promise for the development of pH‐targeting therapies, either against pH regulators or sensors to inhibit cell proliferation and migration.

---

---

Metadata last modified: 26 Jan 2021 17:56

Owner only: item control page

Download Statistics

Download Statistics

Download Statistics

Download Statistics

Downloads

Downloads per month over past year

Altmetric

Altmetric

Altmetric

Alternative Statistics (altmetrics)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)

More literature (via CORE)