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Increasing the throughput of label-free cell assays to study the activation of G-protein-coupled receptors by using a serial agonist exposure protocol

Stolwijk, J. A. ; Skiba, M. ; Kade, C. ; Bernhardt, G. ; Buschauer, Armin ; Hübner, H. ; Gmeiner, P. ; Wegener, Joachim



Abstract

Label-free, holistic assays, monitoring, for example, the impedance of cells on electrodes, are gaining increasing popularity in the evaluation of G-protein-coupled receptor (GPCR) ligands. It is the strength of these approaches to provide the integrated cellular response non-invasively, highly automated and with a device-dependent time resolution down to several milliseconds. With an increasing ...

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