a, Flies expressing an RNAi construct targeting the FoxP isoform B were impaired in operant self-learning, compared to the genetic controls (pooled, see Materials and Methods; Mann-Whitney U-Test, U = 378.5, p<0.03) in a 2-minute test immediately after eight minutes of training. See raw data or Fig. S2 for separated control groups.

b, Both flies expressing an RNAi construct targeting the FoxP isoform B, as well as genetic control flies performed well in operant world-learning (Mann-Whitney U-Test, U = 420.5, P<0.2).

c, No reduction in the expression of isoform B mRNA using qRT-PCR in flies expressing an RNAi construct targeting the FoxP isoform B. Canton S data are identical to those in Fig. 2c, as experiments were performed simultaneously.

d, Mutant FoxP³⁹⁵⁵ flies were impaired in habit formation. In a 2-minute self-learning test (i.e. without colors) after 16 minutes of training in world-learning (i.e. with colors), FoxP³⁹⁵⁵ flies showed a significantly reduced preference for the previously unpunished turning-maneuvers, compared to wild type control animals (Mann-Whitney U-Test, U = 186.0, p<0.04). However, the mutant flies nevertheless showed a significant performance index (t-test against zero: df: 23; t = 2.54; p<0.02).